Surface hardness and color change of dental enamel exposed to cigarette smoke

Carlos Eduardo dos Santos Bertold, Diogo de Azevedo Miranda, Eduardo José Souza-Junior, Flávio Henrique Baggio Aguiar, Débora Alves Nunes Leite Lima, Rodrigo Lessa Ferreira, Igor Roberto Claes, José Roberto Lovadino


Background: Tooth susceptibility to discoloration can be attributed to defects in enamel and dentin due to pigments penetration into the cracks and tubules present in the dental substrates Aims & Objectives: This in vitro study was undertaken to evaluate the microhardness and the color change of dental enamel exposed to cigarette smoke. Materials & Methods: Fifteen bovine enamel fragments (25mm2) were submitted to exposure to the equivalent of cigarette smoke from 50 cigarettes over a period of five days. Initial and final readings of microhardness and colorimetric evaluation were performed. The results were analyzed statistically with the one-way ANOVA and Tukey test (p≤0.05). Results: The samples presented a significant color change and a decrease in the values of microhardness. Conclusion: In conclusion, the exposure to cigarette smoke can significantly alter the microhardness of enamel and its coloring.



Knoop hardness, Color, cigarette smoke, dental enamel


Kleter GA, Damen JJ, Buijs MJ, Ten Cate JM. Modification of amino acid residues in carious dentin matrix. Journal of Dental Research 1998;77(3):488-95.

Polydorou O, Mönting JS, Hellwig E, Auschill TM. Effect of in-office tooth bleaching on the microhardness of six dental esthetic restorative materials. Dental Materials 2007;23(2): 153-8.

Schulze KA, Marshall SJ, Gansky SA, Marshall GW. Color stability and hardness in dental composites after accelerated aging. Dental Materials 2003;19(7):612-9.

Bagheri R, Burrow MF, Tyas M. Influence of food-simulating solutions and surface finish on susceptibility to staining of aesthetic restorative materials. Journal of Dentistry 2005;33(5):389-98.

Satou N, Khan AM, Matsumae I, Satou J, Shintani H. In vitro color change of composite-based resins. Dental Materials 1989;5(6):384-7.

Raptis CN, Powers JM, Fan PL, Yu R. Staining of composite resins by cigarette smoke. Journal of Oral Rehabilitation 1982;9(4):367-71.

Rossie KM, Guggenheimer J. Thermally induced 'nicotine' stomatitis. A case report. Oral Surgery Oral Medicine Oral Pathology 1990;70(5):597-9.

Caldwell RC, Muntz ML, Gilmore RW, Pigman W. Microhardness studies of intact surface enamel. Journal of Dental Research 1957;36(5):732-8.

Newbrun E, Timberlake P, Pigman W. Changes in microhardness of enamel following treatment with lactate buffer. Journal of Dental Research 1959;38(2):293-300.

Lima DA, Silva AL, Aguiar FH, Liporoni PC, Munin E, Ambrosano GM, Lovadino JR. In vitro assessment of the effectiveness of whitening dentifrices for the removal of extrinsic tooth stains. Brazilian Oral Research 2008;22(2):106-11.

Fragoso LS, Lima DA, de Alexandre RS, Bertoldo CE, Aguiar FH, Lovadino JR. Evaluation of physical properties of enamel after microabrasion, polishing, and storage in artificial saliva. Biomedical Materials 2011;6(3):035001.

Lee YK, Lim BS, Powers JM. Color changes of dental resin composites by a salivary enzyme. Journal of Biomedical Materials Research Part B Applied Biomaterials 2004 ;70(1):66-72.

Buchalla W, Attin T, Hilgers RD, Hellwig E. The effect of water storage and light exposure on the color and translucency of a hybrid and a microfilled composite. Journal of Prosthetic Dentistry 2002;87(3):264-70.

Takeuchi CY, Corrêa-Afonso AM, Pedrazzi H, Dinelli W, Palma-Dibb RG. Deposition of lead and cadmium released by cigarette smoke in dental structures and resin composite. Microscopy Research and Technique 2010.

Palamara J, Phakey PP, Rachinger WA, Orams HJ. The ultrastructure of human dental enamel heat-treated in the temperature range 200 degrees C to 600 degrees C. Journal of Dental Research 1987;66(12):1742-7.

Craig RG & Powers JM. 2002. Restorative dental materials. St. Louis: Mosby.

Joiner A. Tooth colour: a review of the literature. Journal of Dentistry 2004;32 Suppl 1:3-12.

Wasilewski Mde S, Takahashi MK, Kirsten GA, de Souza EM. Effect of cigarette smoke and whiskey on the color stability of dental composites. American Journal of Dentistry 2010;23(1):4-8.

Sulieman M. An overview of tooth discoloration: extrinsic, intrinsic and internalized stains. Dental Update 2005;32(8):463-4, 466-8, 471.

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