Comparative analysis of periodontal pathogens in smokers and non-smokers with chronic periodontitis - A Microbiological Study
Aims: To compare the periodontal pathogens in smokers and non-smokers using, a non-invasive chair side technique, BANA (N-benzoyl- DL- arginine 2- napthylamide). Materials and Method: A total of 30 men were randomly selected out of which 15 are smokers and the rest 15 are non-smokers. Thirty sites in smokers and thirty sites in nonsmokers were selected for the study. Subgingival plaque samples from the selected sites in both Smokers and non-Smokers were subjected to microbial examination. Results: Out of the 30 sites examined in smokers, (48.3%) showed BANA positive reactions compared to (16.7%) in non-smokers. Papillary bleeding score was taken in 30 sites in smokers and non-smokers. Similarly in our study Smokers with papillary bleeding score 3 had more BANA positive species (53.3%) than non-Smokers (26.7%), Conclusion: In conclusion the study result shows that there were more BANA positive species in smokers than non-smokers which is more significant (P -0.02%) and papillary bleeding score increases with BANA positive species.
Chaudhary H, Shetty S, Sharda AJ, Agarwal AA, Singh KP. Effect of smoking status in support to anti-smoking legislation among medical and dental professionals in an Indian City-a Cross Sectional Study. International Journal of Dental Clinics. 2010;2(4).
Nadeem M, Arshad S, Abid M. Association between smoking and Intra-oral distribution of dental plaque pattern in a Pakistan population. International Journal of Dental Clinics. 2012;4(1).
Nadeem M, Shafique R, Yaldram A, López R. Intraoral distribution of oral melanosis and cigarette smoking in a Pakistan population. International Journal of Dental Clinics. 2011;3(1).
MacFarlane GD, Herzberg MC, Wolff LF, Hardie NA. Refractory periodontitis associated with abnormal polymorphonuclear leukocyte phagocytosis and cigarette smoking. Journal of periodontology. 1992;63(11):908-13.
Pabst MJ, Pabst KM, Collier JA, Coleman TC, Lemons-Prince ML, Godat MS, et al. Inhibition of neutrophil and monocyte defensive functions by nicotine. Journal of Periodontology. 1995;66(12):1047-55.
Persson L, Bergström J, Gustafsson A, Asman B. Tobacco smoking and gingival neutrophil activity in young adults. Journal of clinical periodontology. 1999;26(1):9-13.
Boström L, Linder LE, Bergström J. Smoking and crevicular fluid levels of IL‐6 and TNF‐a in periodontal disease. Journal of Clinical Periodontology. 1999;26(6):352-7.
Tangada SD, Califano JV, Nakashima K, Quinn SM, Zhang J-B, Gunsolley JC, et al. The Effect of Smoking on Serum IgG2 Reactive With Actinobacillus actinomycetemcomitans in Early-Onset Periodontitis Patients. Journal of periodontology. 1997;68(9):842-50.
Armitage GC. Periodontal diseases: diagnosis. Annals of periodontology/the American Academy of Periodontology. 1996;1(1):37.
Bretz W, Loesche W. Characteristics of trypsin-like activity in subgingival plaque samples. Journal of dental research. 1987;66(11):1668-72.
Schmidt E, Bretz W, Hutchinson R, Loesche W. Correlation of the hydrolysis of benzoyl-arginine naphthylamide (BANA) by plaque with clinical parameters and subgingival levels of spirochetes in periodontal patients. Journal of dental research. 1988;67(12):1505-9.
Preber H, Kant T, Bergström J. Cigarette smoking, oral hygiene and periodontal health in Swedish army conscripts. Journal of clinical periodontology. 1980;7(2):106-13.
Feldman RS, Bravacos JS, Rose CL. Association between smoking different tobacco products and periodontal disease indexes. Journal of Periodontology. 1983;54(8):481-7.
Markkanen H, Paunio I, Tuominen R, Rajala M. Smoking and periodontal disease in the Finnish population aged 30 years and over. Journal of dental research. 1985;64(6):932-5.
Bergström J, Eliasson S. Cigarette smoking and alveolar bone height in subjects with a high standard of oral hygiene. Journal of Clinical Periodontology. 1987;14(8):466-9.
Bergström J, Preber H. The influence of cigarette smoking on the development of experimental gingivitis. Journal of Periodontal Research. 1986;21(6):668-76.
Kinane D, Radvar M. The Effect of Smoking on Mechanical and Antimicrobial Periodontal Therapy. Journal of Periodontology. 1997;68(5):467-72.
Zambon J, Grossi S, Machtei E, Ho A, Dunford R, Genco R. Cigarette Smoking Increases the Risk for Subgingival Infection With Periodontal Pathogens. Journal of periodontology. 1996;67(10s):1050-4.
Sayers NM, James JA, Drucker DB, Blinkhorn AS. Possible potentiation of toxins from Prevotella intermedia, Prevotella nigrescens, and Porphyromonas gingivalis by cotinine. Journal of periodontology. 1999;70(11):1269-75.
Bretz W, Lopatin D, Loesche W. Benzoyl‐arginine naphthylamide (BANA) hydrolysis by Treponema denticola and/or Bacteroides gingivalis in periodontal plaques. Oral microbiology and immunology. 1990;5(5):275-9.
de Moraes GRISI MF, Novaes AB, Ito IY, de Souza SALVADOR SL. Relationship between clinical probing depth and reactivity to the BANA test of samples of subgingival microbiota from patients with periodontitis. Braz Dent J. 1998;9(2):77-84.
Loesche W, Syed S, Stoll J. Trypsin-Like Activity in Subgingival Plaque* A Diagnostic Marker for Spirochetes and Periodontal Disease? Journal of periodontology. 1987;58(4):266-73.
Rivera-Hidalgo F. Smoking and periodontal disease: a review of the literature. Journal of periodontology. 1986;57(10):617-24.
Feitosa A, Amalfitano J, Loesche W. The effect of incubation temperature on the specificity of the BANA (N‐benzoyl‐DL‐arginine‐naphthylamide) test. Oral microbiology and immunology. 1993;8(1):57-61.
Kamma JJ, Nakou M, Baehni P. Clinical and microbiological characteristics of smokers with early onset periodontitis. Journal of periodontal research. 1999;34(1):25-33.
Darby I, Hodge P, Riggio M, Kinane D. Microbial comparison of smoker and non‐smoker adult and early‐onset periodontitis patients by polymerase chain reaction. Journal of Clinical Periodontology. 2000;27(6):417-24.
Kasuga Y., Isgihara K, Okuda K. Significance of Detection of Porphyromonas gingivalis, Bacteroides forsythus and Treponema denticola in Periodontal Pockets. The Bulletin of Tokyo Dental College. 2000;41(3):109-17.
- There are currently no refbacks.
|Published by Celesta Software Pvt Ltd|